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Immobilization of compounds with COOH groups

Immobilization to NH2beads

Compounds with COOH radicals can be bound to NH2 beads. Using a condensing agent (HOSu, HOBt, DMAP), an amide bond forms between the COOH radicals of the compound and the NH2 radicals of the beads. If there are remaining NH2 radicals on the FG bead surfaces, then the surface of the FG beads® becomes charged and electrostatic protein adsorption will occur. Therefore, it is necessary to mask the remaining NH2 radicals with acetic anhydride.

No.Protocol
005Immobilization of ligands (carboxylic compounds) on NH2 beads

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Immobilization to OH beads

If the reactivity of the compound COOH radicals is high, it is possible to bind the compound to OH beads. Using a condensing agent, ester bonds are formed between the COOH radicals of the compound and the OH radicals of the beads. Because OH beads undergo produce no non-specific protein adsorption, it is not necessary to mask the remaining OH radicals.

No.Protocol
004Immobilization of ligands (carboxylic compounds) on OH beads

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