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Protocol download

We offers protocols about immobilization of ligands and purification of proteins etc.

Chemical pulldown

No.Protocolファイル
001Screening by using ligand immobilized beads Screening 101KB
003Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads EpoxybeadsへのPhenol group・Amino groupLigandのImmobilization 90KB
004Immobilization of ligands (carboxylic compounds) on OH beads OHbeadsへのCOOhLigandのImmobilizationHOSu 67KB
005Immobilization of ligands (carboxylic compounds) on NH2 beads NH2beadsへのCOOHLigandのImmobilizationHOSu 95KB
008Immobilization of ligands (compounds with NH2 groups) on COOH beads COOHbeadsへのNH2LigandのImmobilization 133KB
012Competitive inhibition Competitive inhibition 103KB
013Drug elution Drug elution 101KB
014Immobilization of ligands (compounds with NH2 groups) on NHS beads NHSbeadsへのNH2LigandのImmobilization 88KB
108Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads NH2beadsへのCOOHLigandのImmobilizationHOSu 93KB
109Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction 74KB
111Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction Immobilization of ligands (azido structure compounds) on azide beads using click chemistry reaction 70KB
201Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) 2,345KB
Ligand immobilization in chemical biology is 2.5 mg / condition in the above protocol. Below is a small scale of 1 mg / condition.
No.プロトコールファイル
003Smmobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads エポキシビーズへのリガンド(フェノール性OH基またはアミノ基を有する化合物)の固定化 90KB
004SImmobilization of ligands (carboxylic compounds) on OH beads OHビーズへのCOOHリガンドの固定化 67KB
005SImmobilization of ligands (carboxylic compounds) on NH2 beads NH2ビーズへのCOOHリガンドの固定化HOSu 95KB
008SImmobilization of ligands (compounds with NH2 groups) on COOH beads化 COOHビーズへのNH2リガンドの固定化 133KB
014SImmobilization of ligands (compounds with NH2 groups) on NHS beads NHSビーズへのNH2リガンドの固定化 88KB
015SImmobilization of MTX aminated derivatives on NHS beads NHSビーズへのMTXアミノ化誘導体の固定化 70KB
109SImmobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction クリックケミストリーを用いたアジドビーズへのリガンドの固定化 244KB
111SImmobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction クリックケミストリー反応を用いたアルキンビーズへのリガンド(アジド構造をもつ化合物)の固定化 244KB

Immunoprecipitation、Protein-protein Interaction

No.Protocolファイル
011Immunoprecipitation Immunoprecipitation using antibody immobilized FG beads<sup>®</sup>100KB
101Immobilization of proteins on COOH beads COOHbeadsへのImmobilization of proteins 89KB
102Immobilization of His-Tag proteins on Ts beads Immobilization of His-Tag proteins on Ts beads 96KB
105Immobilization of Antibodies or Proteins on NHS beads NHSbeadsへのImmobilization of proteins or antibodies 80KB
106Immobilization of Antibodies or Proteins on Epoxy Beads EpoxybeadsへのImmobilization of proteins 78KB
107Direct Quantification of Immobilized Proteins (Antibodies) 固定化されたタンパク質(抗体)固定化量の定量 102KB
108Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads NH2beadsへのCOOHLigandのImmobilizationHOSu 93KB
110Immobilization of antibodies on Protein A beads and Protein G beads 134KB

Purification of DNA and RNA binding substances

No.Protocolファイル
001Screening by using ligand immobilized beads Screening 101KB
301Immobilization of double strand DNA on Plain beads Plain beadsへのDNAのImmobilization 137KB

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